Research Article
Differences and Similarities in the Metabolism of Glyburide for Various Species: An Analysis by LC-DAD-Q-TRAP-MS/MS
Selvan Ravindran*, Santosh Kapil Kumar Gorti, Sudipta Basu, Prashant Surve and Pradnya Honrao
Department of Biotransformation, Drug Metabolism and Pharmacokinetics Unit, Sai Life Sciences Limited, Pune, India
- *Corresponding Author:
- Selvan Ravindran, Ph.D.
Department of Biotransformation
Drug Metabolism & Pharmacokinetics Unit
Sai Life Sciences Limited
International Bio-Tech Park
Phase-II, Hinjewadi, Pune-411 057, India
Tel: +91 20 6674 3600
Fax: +91 20 6674 3645
E-mail: selvan.r@sailife.com, selvan_ravindran@yahoo.com
Received date: January 30, 2013; Accepted date: March 15, 2013; Published date: March 18, 2013
Citation: Ravindran S, Kumar Gorti SK, Basu S, Surve P, Honrao P (2013) Differences and Similarities in the Metabolism of Glyburide for Various Species: An Analysis by LC-DAD-Q-TRAP-MS/MS. J Anal Bioanal Tech 4:164. doi: 10.4172/2155-9872.1000164
Copyright: © 2013 Ravindran S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the riginal author and source are credited.
Abstract
Studies were conducted on the of sulfonylurea drug glyburide (5-chloro-N-(4-[N-(cyclohexylcarbamoyl) sulfamoyl] phenethyl)-2-methoxybenzamide) in human, mouse, rat, dog and monkey hepatic microsomes. Liquid chromatography with Diode array detector (LC-DAD) hyphenated with Q-Trap-Mass Spectrometer (Q-TRAP-MS/ MS) was employed to study the metabolism of glyburide in different species. The primary objective of the present study is to identify the similarities and differences in the metabolism of glyburide and to confirm the recent newly identified metabolites across the species. Results obtained from LC-UV and LC-MS/MS confirm the similarities and differences in the biotransformation of glyburide across the species. LC-UV-MS/MS data clearly suggests that the quantities of metabolites formed in all the species are dissimilar. Drug metabolite ratio is also different in all the species considered for tests. Mono-oxygenated metabolites and a metabolite due to the ring loss were identified in all the species. Similarities and differences in the metabolism of glyburide confirm the role of cytochrome P450 (CYP 45) enzymes and its distinct activity across the species.
