91桃色

Abstract

Tuberculosis (TB) continues to be a major global health problem, with the emergence and spread of Multidrug-Resistant Tuberculosis (MDR-TB). Genetic factors play a significant role in determining susceptibility to TB and the development of drug resistance. Brazil is in one of the most genetically diverse populations in the world due to the high mix between Amerindian, African and European populations, which makes this population unique in relation to the world for genomic studies. This study aimed to investigate the genotypic differences in MDR-TB-associated variants and their potential implications as genetic markers for these conditions in a Brazilian Amazon population with a high degree of admixture. This case-control study was conducted on 190 subjects, including 165 TB patients and 25 MDR-TB patients. The case group was composed of individuals diagnosed with MDR-TB, while the control group was composed of TB patients at the João Barros Barreto Hospital, in Belém (Pará-Basil). Statistical tests revealed significant differences in African ancestry between MDR-TB groups. The results regarding the investigated SNVs were significant for the risk of developing MDR-TB for mRNA: miR453 rs56103835, miR608 rs4919510, miR499 rs3746444 and miR423 rs6505162. These Single-Nucleotide Polymorphism (SNVs) should be better investigated in subsequent studies with larger populations, as the population found in this study was small, which may have limited the causal relationships; in this way, new research can map populations susceptible to severe forms of tuberculosis. With the aim of applying new, more effective and individualized diagnostic means, based on genomics, contributing to reducing the socioeconomic impact in countries affected by forms of multidrug-resistant tuberculosis, equipping governments and public policies to identify susceptible people, monitor the treatment and avoid wasting time and resources.

Keywords: Genetic variation; Mix population; Multidrug-resistant tuberculosis