Research Article
Rapid Measurement of Adenosine Concentration in Human Blood Using Fixed Potential Amperometry: Comparison with Mass Spectrometry and High- Performance Liquid Chromatography
Marion Marlinge1, Donato Vairo2, Viviana Marolda2, Laurie Bruzzese2, Nabil Adjriou2, Claire Guiol2, Nathalie Kipson2, Anna Bonnardel2, Marguerite Gastaldi1, François Kerbaul3, Pierre Michelet3, Jean Claude Deharo4, Giovanna Mottola2, Patrick Mace1, Mohamed Chefrour1 and Regis Guieu1,2*1Laboratory of Biochemistry, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France
2UMR MD2, Aix Marseille University, Bd Pierre Dramard, 13015 Marseille, France
3RUSH Department, Timone 2, 264 Rue Saint-Pierre, 13385 Marseille, France
4Department of Cardiology, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France
- *Corresponding Author:
- Régis Guieu
UMR MD2, Aix Marseille University
Bvd P Dramard 13015, Marseille
France
Tel: +33491396500
E-mail: guieu.regis@orange.fr
Received date: July 11, 2017; Accepted date: July 17, 2017; Published date: July 20, 2017
Citation: Marlinge M, Vairo D, Marolda V, Bruzzese L, Adjriou N, et al. (2017) Rapid Measurement of Adenosine Concentration in Human Blood Using Fixed Potential Amperometry: Comparison with Mass Spectrometry and High-Performance Liquid Chromatography. J Anal Bioanal Tech 8: 371. doi: 10.4172/2155-9872.1000371
Copyright: © 2017 Marlinge M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA), with usual methods: mass spectrometry (LC-MS/MS) or high performance liquid chromatography (HPLC). Methods: Twenty healthy subjects (14 men and 6 women) and ten patients suffering from vasovagal syncope (VVS, 6 women and 4 men) were included. Blood samples were collected by vein puncture for plasma adenosine assay and in the same time using finger puncture for direct FAP measurement and on blotting paper for LC-MS/MS. Results: Mean plasma adenosine concentration was 26% higher using HPLC compared with LC-MSMS; p<0.01. In whole blood, adenosine concentration was 35% higher using FPA compared with LC-MS/MS. We found a good correlation between adenosine values measured by FAP and LC-MS/MS in whole blood and between LCMS/ MS and HPLC in plasma. Mean adenosine concentration was higher in patients whatever the method used. Conclusion: Adenosine measurement to the patient’s bed, using FPA may be useful in some cases where high adenosine is associated with pejorative outcome.
