Research Article
Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata)
Geetika Gambhir* and Srivastava DKDepartment of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Himachal Pradesh, India
- Corresponding Author:
- Geetika Gambhir
Department of Biotechnology
Dr. Y.S. Parmar University of Horticulture and Forestry
Solan-173230, Himachal Pradesh, India
E-mail: geetikabt@gmail.com/dksuhf89@gmail.com
Received date: January 16, 2015; Accepted date: March 13, 2015; Published date: March 20, 2015
Citation: Gambhir G, Srivastava DK (2015) Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata). J Biotechnol Biomater 5:172. doi:10.4172/2155-952X.1000172
Copyright: © 2015 Gambhir G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
We have studied the effect of thidiazuron on shoot regeneration from leaf and petiole explants of cabbage (Brassica oleracea L. var. capitata cv. Pride of India). Different concentrations and combinations of thidiazuron (alone), thidiazuron+ Adenine, thidiazuron+ NAA and thidiazuron+ IAA were used in MS medium to optimize in vitro shoot regeneration from leaf and petiole explants of cabbage (Brassica Oleracea L. var. capitata cv. Pride of India). Leaf and petiole explants procured from 20-25 days old glasshouse grown seedlings of cabbage. Out of 40 combinations and concentrations tried separately for leaf and petiole explants, the high frequency shoot regeneration from leaf (91.11%) and petiole (88.88%) explant was obtained on MS medium supplemented with0.220mg/l thidiazuron + 0.02 mg/l NAA and 0.330 mg/l thidiazuron + 0.02 mg/l NAA respectively. High percentage root regeneration (100%) in in vitro developed shoots was obtained on MS medium supplemented with 0.10mg/l NAA. The regenerated complete plantlets were transferred to pots containing sterilized coco peat and acclimatized. A high efficiency and reliable plant regeneration protocol has been developed using thidiazuron. This regenerated protocol would be useful for genetic transformation studies in cabbage.
