ISSN: 2155-6199
Journal of Bioremediation & Biodegradation
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Assessment of bioplastic producing potential of Bacillus subtilis using some agro residues as carbon source
3rd International Conference and Exhibition on Biopolymers & Bioplastics
Abdullahi B Sallau and Bashir Salim
Ahmadu Bello University Nigeria
Posters & Accepted Abstracts: J Bioremed Biodeg
DOI:
Abstract
The assessment of bioplastic producing potential of Bacillus subtilis using a variety of pretreated agro-residues was carried out. The agro residues were rice husks, molasses, bagasse, and corn cobs. Acid, alkaline and oxidative pretreatment of the agro-residues were done using standard procedures. Bioplastics produced were extracted using chloroform precipitation and quantified spectrophotometer. Acid, base and peroxide concentrations in g/L of polyhydroxybutyrate (PHB) were obtained for rice husks 1.52�?卤0.02, 1.82�?卤0.01, and 1.70�?卤0.01; molasses 1.82�?卤0.01, 1.52�?卤0.02, and 1.69�?卤0.01; bagasse 0.87�?卤0.06, 1.10�?卤0.10, and 0.96�?卤0.07 and; corn cobs 0.5�?卤0.00, 0.77�?卤0.06, and 0.60�?卤0.10. The bioplastic yield of the agro-residues for acid, base and peroxide pretreatments were: rice husks 50.33�?卤0.76, 53.69�?卤2.23 and 53.29�?卤0.15; molasses 54.94�?卤0.14, 50.55�?卤0.25 and 53.09�?卤0.17%; bagasse 53.33�?卤6.68, 55.00�?卤0.25 and 54.50�?卤7.12 and; corn cobs 51.85�?卤3.21, 63.94�?卤2.59 and 60.11�?卤8.89. Statistical analysis revealed that PHB concentration of alkaline pretreated rice husk had a significant (p<0.05) higher value than other pretreatments. However, among all the agro-residues used peroxide pretreated corn cobs had a significantly (p<0.05) higher percentage yield of bioplastic (63.94�?卤2.59).Biography
Email: sallauabdullahi@gmail.com
