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Exopolysaccharide production, related gene alr2882 expression and chemical characterization using FTIR in Anabaena sp. PCC 7120 under different CaCl2 regimes
6th World Congress on Biotechnology
Savita Singh and A K Mishra
Banaras Hindu University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
Cyanobacterial EPS offer much advantage over EPS from other microbial origin. These polysaccharides protect microbial cells from dessication, penetration of toxic metals, antibiotic, phagocytosis, phage attack, salinity and many more stresses. In lieu of this, EPS isolation, production and its chemical characterization using FTIR along with expression analysis of alr2882 encoding gene product ExoD through RT-PCR under different calcium chloride regimes have been investigated. SEM of cyanobacterial suggested that amount of EPS synthesis depends on the concentration of calcium ions in the immediate environment. EPS production was maximum at 10 mM CaCl2 concentration, so was the expression of alr2882. FTIR analysis showed shift in the major band suggesting clearly that calcium levels in the external environment lead to change in the structure of EPS produced by the Anabaena sp. PCC 7120. Thus, the present findings may have fair possibilities of EPS with novel features that may offer biotechnological/industrial applications.Biography
Savita Singh, a PhD student from Department of Botany, Banaras Hindu University specializes in the field of Stress Biology of Cyanobacteria.
Email: singhsavita3@gmail.com
