Journal of Fisheries & Livestock Production
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Hemoglobin from the blood clam Tegillarca granosa (Tg-HbIIA, Tg-HbIIB): Expression and antibacterial activity of recombinant proteins
6th Global Summit on Aquaculture and Fisheries 2017
Yongbo Bao and Danli Song
Zhejiang Wanli University, China
Posters & Accepted Abstracts: J Fisheries Livest Prod
DOI:
Abstract
The hemoglobins produced by Tegillarca granosa have antibacterial activity toward some Gram-positive and Gram-negative bacteria. In this study, the genes encoding the recombinant proteins Tg-HbIIA and Tg-HbIIB were cloned from T. granosa hemocytes by RT-PCR, and the proteins were expressed in Escherichia coli Transetta (DE3). The proteins were purified using a HisTrap FF affinity chromatography column under denaturing conditions and refolded at 4 �?掳C by urea gradient dialysis, and the antibacterial activity of the recombinant proteins was determined. The Tg-HbIIA protein had antibacterial activity toward Vibrio harveyi and Pseudomonas putida, with the minimum inhibition concentration (MIC) values of 6.58�?�?10-2 mg/ml and 4.11�?�?10-3 mg/ml, respectively. The Tg-HbIIB protein had antibacterial activity toward V. harveyi, P. putida and Acinetobacter baumanii, with MIC values of 1.58�?�?10-1 mg/ml, 3.95�?�?10-2 mg/ml and 7.90�?�?10-2 mg/ml, respectively. They had no antibacterial activity against Staphylococcus aureus, E. coli, Bacillus firmus, B. subtilis, S. epidermidis or V. parahaemolyticus. This study provides a basis for further research on the antibacterial function and mechanism of hemoglobin.Biography
Yongbo Bao has expertise in three field of his research: (1) Immune mechanism of molluscs: Molluscan immune and disease-resistant gene function, immune mechanism, antibacterial peptides and antibacterial mechanism etc. (2) Mollucan genetics and molecular assistant breeding: Population genetic structure, genetic linkage map, SNP and GWAS and phenotype association analysis. (3) Gene and molecular phylogeny and evolution: Immunity and development related gene function study and molecular evolution analysis.
Email: bobbao2001@gmail.com
