91ÌÒÉ«

Abstract

Population growth, industrial development, intensified agricultural production and the number of contaminants incorporated into the environment have deteriorated the water compartments, it is necessary to develop strategies to reduce and prevent their contamination. In some types of wastewater, products resulting from the biological treatment process (non-biodegradable) may have significant toxicity and high genotoxicity. Genotoxicological effects may manifest at different levels, from subcellular structures or enzyme systems, to whole organisms. The unicellular electrophoresis/Comet assay (EC) and micronucleus are sensitive methodologies, available, cheap and applicable to any cell type to measure DNA strand breaks in individual cells considered as assays indicative of premutagenic lesions. The evaluation of abnormal cells was performed in producers using treated wastewater for agricultural irrigation. The presence of nuclear and micronuclei abnormalities in the identified cells was classified in relation to their nuclear form by observing degenerative nuclear changes related to cellular toxicity. Among the nuclear abnormalities detected in the producers prevail the condensed and binucleated cell nuclei, without observation of more than six micronuclei per thousand cells counted; however, tests on bioindicators (Allium sativum and Vicia faba) will be carried out to confirm genotoxicity levels generated by the compounds present in the wastewater.

Biography

Perez-Nava Jessica has completed her MSc from Benémerita Universidad Autónoma de Puebla. Currently, she is pursuing her Doctoral program in the same university. Her research areas include: photochemical treatment of wastewater, genotoxicity and microbiological tests. She has published two scientific papers and has made academics mobilities in Mexico and abroad.
Email:jesyolpris_1@hotmail.com