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Journal of Biotechnology & Biomaterials
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Production of extra cellular 1-4-beta-D-endo xylanase by Bacillus brevis using agro-waste
Industrial Biotechnology Congress
Girish K Goswami
C U Shah University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
The xylanase biosynthesis is induced by its substrate-xylan. For the cost effective production of endo-xylanase, an enzyme of immense industrial importance, xylanase production from Bacillus brevis was studied. The high xylan content in some of the wastes like rice bran, wheat bran, rice straw and wheat straw makes them an accessible and cheap source of inducers. Culture medium for xylanase biosynthesis at shake flask has been optimized. Xylanase activity was measured by DNSA methods using birchwood xylan (Sigma-Aldrich,USA) as standard substrate. The substrate optimization process was analyzed using agro-waste concentration from 0.5-10% (w/v). Maximum xylanase activity was observed on media supplemented with 2% of agro-waste. Wheat bran was found to be the best producer for xylanase production out of other substrates tried out viz. Rice bran, wheat bran, rice straw and wheat straw with xylanase activity 39 IU/ml. Optimum incubation temperature of B. brevis xylanase was found to be 72�?掳 C at pH 7.0.Biography
Email:
girishkgoswami@gmail.com